Segmentation of Drosophila Heart in Optical Coherence Microscopy Images Using Convolutional Neural Networks
نویسندگان
چکیده
Convolutional neural networks are powerful tools for image segmentation and classification. Here, we use this method to identify and mark the heart region of Drosophila at different developmental stages in the cross-sectional images acquired by a custom optical coherence microscopy (OCM) system. With our well-trained convolutional neural network model, the heart regions through multiple heartbeat cycles can be marked with an intersection over union (IOU) of ~86%. Various morphological and dynamical cardiac parameters can be quantified accurately with automatically segmented heart regions. This study demonstrates an efficient heart segmentation method to analyze OCM images of the beating heart in Drosophila. References and links 1. D. Huang, E. Swanson, C. Lin, J. Schuman, W. Stinson, W. Chang, M. Hee, T. Flotte, K. Gregory, C. Puliafito, and a. et, "Optical coherence tomography," Science 254, 1178-1181 (1991). 2. W. Drexler, M. Liu, A. Kumar, T. Kamali, A. Unterhuber, and R. A. Leitgeb, "Optical coherence tomography today: speed, contrast, and multimodality," Journal of Biomedical Optics 19, 071412 (2014). 3. J. Fujimoto and E. Swanson, "The Development, Commercialization, and Impact of Optical Coherence Tomography," Investigative Ophthalmology & Visual Science 57, OCT1-OCT13 (2016). 4. M. Wojtkowski, "High-speed optical coherence tomography: basics and applications," Appl. Opt. 49, D30-D61 (2010). 5. A. D. Aguirre, C. Zhou, H.-C. Lee, O. O. Ahsen, and J. G. Fujimoto, "Optical Coherence Microscopy," in Optical Coherence Tomography: Technology and Applications, W. Drexler and J. G. Fujimoto, eds. (Springer International Publishing, Cham, 2015), pp. 865-911. 6. J. A. Izatt, M. R. Hee, G. M. Owen, E. A. Swanson, and J. G. Fujimoto, "Optical coherence microscopy in scattering media," Opt. Lett. 19, 590-592 (1994). 7. L. Kagemann, H. Ishikawa, J. Zou, P. Charukamnoetkanok, G. Wollstein, K. A. Townsend, M. L. Gabriele, N. Bahary, X. Wei, J. G. Fujimoto, and J. S. Schuman, "Repeated, noninvasive, high resolution spectral domain optical coherence tomography imaging of zebrafish embryos," Molecular Vision 14,
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